Engineered Mammalian Cell Lines

Transposase mediated genomic integration 38 39 accelerates the generation of permanently engineered cell lines for implants.

Engineered mammalian cell lines.

For this reason all of the therapeutic proteins produced in mammalian cells employ cell lines. However because they can be cultured forever cell lines can be genetically engineered to produce a product in virtually unlimited quantities. The expi293f inducible cell line is derived from engineered expi293f cells that stably express the tetracycline repressor tetr protein thus enabling tetracycline regulated protein expression. Those systems can even ensure the natural folding and sugar chain composition of glycoprotein and significantly increase the expression level of glycoprotein and.

Mammalian cells exposed to each zfn pair in turn resulted in the generation of cell lines harboring single double and triple gene knockouts that is the successful disruption of two four and. The cell line was established by the stable integration of the regulatory plasmid pcdna6 tr which encodes the tetr gene under the control of the human. This approach also simplifies the use of other cell lines and might ease the transition to cell types that are approved for human therapy 29 40 41.